|
KMID : 1007519960050030226
|
|
Food Science and Biotechnology
1996 Volume.5 No. 3 p.226 ~ p.228
|
|
|
Evaluation of Sandwiched Microtiter Plate (SMP) and Mini - Tube Methods for Recovery of Clostridium perfringens from Foods
|
|
Daniel, Y C
LEE, KE HO/Kang, Dong Hyun/Ji, Geun Eog
|
|
|
Abstract
|
|
|
|
Clostridium perfringens from food samples were counted and tested by the SMP and mini-tube methods and compared with double-tube method. One hundred ㎕ of SFP (Shahidi Ferguson Perfringens) agar and 10 ㎕ of diluted food samples were used in the SMP anaerobic system. The samples were sandwiched between two sterile microtiter plates to create an anaerobic environment. Growth of Clostridium Cultures formed black colonies in the sandwiched wells and were counted. In the case of the mini-tube system, 1㎖ of SFP agar (45℃) containing diluted food sample was aspirated into a 1.2 ㎖ sterile pipette and allowed to solidify. The two ends were sealed to create an anaerobic environment. Black colonies were counted directly in the plastic tube. C. perfringens was inoculated into food samples for recovery studies. Greater numbers of the organisms were recovered in the double-tube system than in the SMP and mini-tube systems after 12 hr incubation. However, the final counts at 24 hr were similar for all methods.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|